ELECTROPHORESIS OF ELASTASE-LIKE ESTERASES FROM HUMAN NEUTROPHILS
نویسندگان
چکیده
منابع مشابه
Elastase-like enzymes in human neutrophils localized by ultrastructural cytochemistry
The thiol ester N-t-Boc-L-alanine-p-nitrothiophenyl ester (Boc-Ala-SNp) was synthesized and applied as an ultrastructural cytochemical substrate for intracellular elastase-like enzymes. Mature human neutrophils incubated with Boc-Ala-SNp and gold ions generate an electron-dense reaction product, gold p-nitrothiophenolate, which is found in the nuclear membrane, Golgi complex, endoplasmic reticu...
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A fast-acting inhibitor of serine elastase has been detected at high levels in human neutrophils, fresh monocytes, matured monocytes, and macrophages. The elastase inhibitor was isolated from large scale cultures of the monocyte-like cell line U937 by DNase chromatography, disulfide exchange, Phenyl-Sepharose, Red A-agarose, and DEAE HPLC chromatography with an average yield of 480 micrograms f...
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Bacterial lipopolysaccharide (LPS) has previously been shown to enhance a number of chemoattractant-induced responses by human neutrophils. The possible role of elastase, a neutral protease with broad substrate specificity, in neutrophil-mediated vascular injury of a variety of diseases prompted us to examine a) whether or not LPS enhances the direct chemoattractant-induced secretion of elastas...
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Esterase activity of the dental pulp has been iernonstrated by histochemical technics on tissue sections by Burstone (Calcification in Biological Systeins, 1960, p. 232) and by Yoshiki (Butt. Tokyo Dent. Coll., 3:14-28, 1962). Aside from the localization of cholinesterases in the human dental pulp by Avery and Rapp (Stain Technol., 33:31-7, 1958) and by Ten Cute and Shelton (Arch. Oral Biol., 1...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1974
ISSN: 0022-1554,1551-5044
DOI: 10.1177/22.5.327